Bacterial populations associated with the oxidation and reduction of arsenic in an unsaturated soil.
نویسندگان
چکیده
Microbial populations responsible for the oxidation and reduction of As were examined in unsaturated (aerobic) soil columns treated with 75 microM arsenite [As(III)] or 250 microM arsenate [As(V)]. Arsenite [As(III)] was rapidly oxidized to As(V) via microbial activity, whereas no apparent reduction of As(V) was observed in the column experiments. Eight aerobic heterotrophic bacteria with varying As redox phenotypes were isolated from the same columns. Three isolates, identified as Agrobacterium tumefaciens-, Pseudomonas fluorescens-, and Variovorax paradoxus-like organisms (based on 16S sequence), were As(III) oxidizers, and all were detected in community DNA fingerprints generated by PCR coupled with denaturing gradient gel electrophoresis. The five other isolates were identified (16S gene sequence) as A. tumefaciens, Flavobacterium sp., Microbacterium sp., and two Arthrobacter sp. -like organisms and were shown to rapidly reduce As(V) under aerobic conditions. Although the two A. tumefaciens-like isolates exhibited opposite As redox activity,their 16S rDNA sequences (approximately 1400 bp) were 100% identical, and both were shown to contain putative arsC genes. Our results support the hypothesis that bacteria capable of either oxidizing As(III) or reducing As(V) coexist and are ubiquitous in soil environments, suggesting that the relative abundance and metabolic activity of specific microbial populations plays an important role in the speciation of inorganic As in soil pore waters.
منابع مشابه
Arsenic Tracking in Iranian Rice: Analysis of Agricultural Soil and Water, Unpolished Rice and White Rice
Since rice is a staple food most consumed world-wide, there have been increasing health concerns regarding exposure to arsenic through rice consumption. Several studies have reported the accumulation of arsenic in rice grains cultivated in regions with elevated levels of arsenic in groundwater or contaminated soil. Therefore the principal aim of this study was determining the amount of arsenic ...
متن کاملAn investigation into a Geocell-reinforced Slope in The Unsaturated Numerical Model
Considering unsaturation conditions of soil significantly helps to produce relatively real results. Numerical methods have been assumed as conventional methods in soil mechanics to examine soil behavior. However, the accuracy of numerical methods dramatically depends on applying the appropriate behavioral model to solve problems. One of the known elastoplastic models for unsaturated soils i...
متن کاملEvaluation of oil pollution dispersion in an unsaturated sandy soil environment
The current study assesses critical condition of oil dispassion, considering the unsaturated soil condition dispersity behavior for oil dispersion. The numerical model is used as a finite element method to model the oil spill pollution with two different saturated and unsaturated soil conditions chosen and their pollution dispersion results compared. Extracted results from numerical model show ...
متن کاملEvaluation of oil pollution dispersion in an unsaturated sandy soil environment
The current study assesses critical condition of oil dispassion, considering the unsaturated soil condition dispersity behavior for oil dispersion. The numerical model is used as a finite element method to model the oil spill pollution with two different saturated and unsaturated soil conditions chosen and their pollution dispersion results compared. Extracted results from numerical model show ...
متن کاملحرکت باکتری اشرشیا کولی (Escherichia coli) آزادشده از کود گاوی در خاک غیراشباع مزرعه
In agriculture, cow manures are used to enhance soil fertility and productivity. Escherichia coli is the most common fecal coliform in cow manure and considered as an index for microbial contamination of groundwater resources. The objective of this study was to investigate the transport of Escherichia coli (released from cow manure) through the field soil. Lysimeters (with internal diameter of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Environmental science & technology
دوره 38 1 شماره
صفحات -
تاریخ انتشار 2004